細(xì)胞名稱

人B淋巴瘤細(xì)胞BJAB

貨物編碼

BFN60806613

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研           2類(lèi)

培養(yǎng)體系

DMEM高糖+10%FBS+1%雙抗

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡(jiǎn)介

人B淋巴瘤細(xì)胞BJAB取自5歲女性供體。懸浮培養(yǎng)。該細(xì)胞源于DSMZ

序列突變

KMT2A-CLTC gene fusion (PubMed=31160637).

TP53 p.His193Arg (c.578A>G) (PubMed=1915267; PubMed=8344493).

注釋

Part of: LL-100 blood cancer cell line panel.

Characteristics: EBV-negative. Even so that the original tumor tissue was infected with the B95-8 strain of EBV.

Doubling time: ~22 hours (PubMed=179629); ~40-60 hours (DSMZ).

Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

STR信息

Amelogenin        X

CSF1PO        8,10

D3S1358        16

D5S818        12,13

D7S820        10,11

D8S1179        15

D13S317        9,11

D16S539        9,11

D18S51        16,22

D21S11        27,28

FGA        27,28

Penta D        10,11

Penta E        7

TH01        7

TPOX        6,9

vWA        14,15 (DSMZ)

14,16 (PubMed=25877200)

參考文獻(xiàn)

PubMed=8515068

Jain V.K., Judde J.-G., Max E.E., Magrath I.T.

Variable IgH chain enhancer activity in Burkitt's lymphomas suggests an additional, direct mechanism of c-myc deregulation.

J. Immunol. 150:5418-5428(1993)

PubMed=8558920

Dirks W.G., Zaborski M., Jager K., Challier C., Shiota M., Quentmeier H., Drexler H.G.

The (2;5)(p23;q35) translocation in cell lines derived from malignant lymphomas: absence of t(2;5) in Hodgkin-analogous cell lines.

Leukemia 10:142-149(1996)

PubMed=23292937; DOI=10.1073/pnas.1205299110

Zhang J., Grubor V., Love C.L., Banerjee A., Richards K.L., Mieczkowski P.A., Dunphy C., Choi W., Au W.Y., Srivastava G., Lugar P.L., Rizzieri D.A., Lagoo A.S., Bernal-Mizrachi L., Mann K.P., Flowers C., Naresh K., Evens A., Gordon L.I., Czader M.B., Gill J.I., Hsi E.D., Liu Q., Fan A., Walsh K., Jima D., Smith L.L., Johnson A.J., Byrd J.C., Luftig M.A., Ni T., Zhu J., Chadburn A., Levy S., Dunson D.B., Dave S.S.

Genetic heterogeneity of diffuse large B-cell lymphoma.

Proc. Natl. Acad. Sci. U.S.A. 110:1398-1403(2013)

PubMed=25485619; DOI=10.1038/nbt.3080

Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.

A comprehensive transcriptional portrait of human cancer cell lines.

Nat. Biotechnol. 33:306-312(2015)

PubMed=31160637; DOI=10.1038/s41598-019-44491-x

Quentmeier H., Pommerenke C., Dirks W.G., Eberth S., Koeppel M., MacLeod R.A.F., Nagel S., Steube K., Uphoff C.C., Drexler H.G.

The LL-100 panel: 100 cell lines for blood cancer studies.

Sci. Rep. 9:8218-8218(2019)